Create a 6-dilution column key for primer calibration
Source:R/plate_functions.R
create_colkey_6diln_2ctrl_in_24.Rd
Creates a 24-column key for primer calibration, with 1x biol_reps and 3x tech_reps, and 5-fold dilution until 5^6 of +RT; then -RT (no reverse transcriptase), NT (no template) negative controls. That is a total of 8 versions of each replicate.
Value
tibble (data frame) with 24 rows, and variables well_col, dilution, dilution_nice, prep_type, biol_rep, tech_rep.
See also
Other plate creation functions:
create_blank_plate()
,
create_colkey_4diln_2ctrl_in_24()
,
create_colkey_6_in_24()
,
create_rowkey_4_in_16()
,
create_rowkey_8_in_16_plain()
,
display_plate_qpcr()
,
display_plate_value()
,
display_plate()
,
label_plate_rowcol()
,
make_row_names_echo1536()
,
make_row_names_lc1536()
Examples
create_colkey_6diln_2ctrl_in_24()
#> # A tibble: 24 × 5
#> well_col dilution dilution_nice prep_type tech_rep
#> <fct> <dbl> <chr> <fct> <fct>
#> 1 1 1 1x +RT 1
#> 2 2 0.2 5x +RT 1
#> 3 3 0.04 25x +RT 1
#> 4 4 0.008 125x +RT 1
#> 5 5 0.0016 625x +RT 1
#> 6 6 0.00032 3125x +RT 1
#> 7 7 1 -RT -RT 1
#> 8 8 1 NT NT 1
#> 9 9 1 1x +RT 2
#> 10 10 0.2 5x +RT 2
#> # ℹ 14 more rows