Function reference
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calculate_deltacq_bysampleid() - Calculate delta cq (\(\Delta Cq\)) to normalize quantification cycle (log2-fold) data within sample_id.
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calculate_deltadeltacq_bytargetid() - Calculate delta delta cq (\(\Delta \Delta Cq\)) to globally normalize quantification cycle (log2-fold) data across sample_id.
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calculate_drdt_plate() - Calculate dR/dT of melt curves for of every well in a plate.
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calculate_dydx() - Calculate dy/dx vector from vectors y and x
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calculate_efficiency() - Calibrate primer sets / probes by calculating detection efficiency and R squared
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calculate_efficiency_bytargetid() - Calibrate multiple probes by calculating detection efficiency and R squared
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calculate_normvalue() - Calculate a normalized value for a subset of reference ids
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create_blank_plate()create_blank_plate_96well()create_blank_plate_1536well() - Create a blank plate template as a tibble (with helper functions for common plate sizes)
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create_colkey_4diln_2ctrl_in_24() - Create a 4-dilution column key for primer calibration
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create_colkey_6_in_24() - Create a 6-value, 24-column key for plates
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create_colkey_6diln_2ctrl_in_24() - Create a 6-dilution column key for primer calibration
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create_rowkey_4_in_16() - Create a 4-value, 16-row key for plates
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create_rowkey_8_in_16_plain() - Create a plain 8-value, 16-row key for plates
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debaseline() - Remove baseline from amplification curves (BETA)
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display_plate() - Display an empty plate plan which can be populated with ggplot2 geom elements.
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display_plate_qpcr() - Display qPCR plate plan with sample_id, target_id, prep_type per well
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display_plate_value() - Display the value of each well across the plate.
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label_plate_rowcol() - Label a plate with sample and probe information
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make_row_names_echo1536() - Generates row names for the Labcyte Echo 1536-well plates
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make_row_names_lc1536() - Generates row names for the Roche Lightcycler (tm) 1536-well plates
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read_lightcycler_1colour_cq() - Reads quantification cycle (cq) data in 1 colour from Roche Lightcyclers
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read_lightcycler_1colour_raw() - Reads raw text-format fluorescence data in 1 colour from Roche Lightcyclers